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KMID : 0043320070300091174
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Archives of Pharmacal Research
2007 Volume.30 No. 9 p.1174 ~ p.1178
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Determination of Eperisone in Human Plasma by Liquid Chromatography-ESI-Tandem Mass Spectrometry
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Jeong Min-Kyo
Jeong Eun-Sook
Kim Nam-Hee
Kim Chang-Soo
Chung Youn-Bok
Lee Yong-Moon
Ahn Su-Youn
Cho Hwang-Eui
Lee Yong-Hwa
Hong Jin-Tae
Moon Dong-Cheul
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Abstract
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A sensitive and selective method for the determination of 4’-ethyl-3-methyl-3-piperidinopropiophenone hydrochloride (eperisone hydrochloride) in human plasma was developed and validated. The procedure employed an internal standard and a solvent extraction step followed by chromatography on a Xterra C18 minibore column. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The mass transitions of eperisone and tolperisone (IS) were m/z 260 → 98 and m/z 246 → 98, respectively. The method has a limit of detection of 0.1 pg/mL for eperisone based on the three times signal-tonoise value with a linear range from 0.01 to 10.0 ng/mL for the analyte. Extraction recovery was on average 98.6±7.2% (SD) for eperisone. The Intra- and inter-day assay accuracy ranged from 93 to 114% and precision (RSD) was better than 8.5%. The method was successfully employed to analyze plasma samples and evaluate pharmacokinetics of eperisone in healthy male volunteers
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KEYWORD
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Eperisone, Tolperisone, Tandem mass spectrometry, Human plasma, Pharmacokinetics
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